Homer2 Blocking Peptide (#BLP-PZ027) is the original antigen used for immunization during Anti-Homer2 Antibody (#APZ-027) generation. The blocking peptide binds and ‘blocks’ Anti-Homer2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):
- Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):
- Expression of Homer2 in mouse cerebellum and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-Homer2 Antibody (#APZ-027), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. Homer2 staining (green) in mouse cerebellum is observed in Purkinje cells (arrows) and their dendrites. B. Homer2 staining (green) in rat hippocampal dentate gyrus region is detected granule layer (GL) and interneurons in the hilus (arrows). Cell nuclei are stained with DAPI (blue).
- (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2 (Accession O88801).