Overview
Type: Synthetic peptide
Form: Lyophilized powder
Homer2 Blocking Peptide (#BLP-PZ027) is the original antigen used for immunization during Anti-Homer2 Antibody (#APZ-027) generation. The blocking peptide binds and ‘blocks’ Anti-Homer2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
3,4. Anti-Homer2 Antibody, preincubated with Homer2 Blocking Peptide (#BLP-PZ027).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
3,4. Anti-Homer2 Antibody, preincubated with Homer2 Blocking Peptide (#BLP-PZ027).
Expression of Homer2 in mouse cerebellum and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-Homer2 Antibody (#APZ-027), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. Homer2 staining (green) in mouse cerebellum is observed in Purkinje cells (arrows) and their dendrites. B. Homer2 staining (green) in rat hippocampal dentate gyrus region is detected granule layer (GL) and interneurons in the hilus (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2 (Accession O88801).
Accession (Uniprot) Number O88801
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
