Homer3 Blocking Peptide (#BLP-PZ028) is the original antigen used for immunization during Anti-Homer3 Antibody (#APZ-028) generation. The blocking peptide binds and ‘blocks’ Anti-Homer3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line (lanes 2 and 4) lysates:
- Expression of Homer3 in rat nucleus basalisImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Homer3 Antibody (#APZ-028), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. Homer3 immunoreactivity (green) appears in cells of the horizontal diagonal band (HDB), (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RSQSADAPGPTERER, corresponding to amino acid residues 155 - 169 of rat Homer3 (Accession Q9Z2X5).