Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human ABCB1/MDR1 Blocking Peptide (#BLP-MT021) is the original antigen used for immunization during Anti-Human ABCB1/MDR1 Antibody (#AMT-021) generation. The blocking peptide binds and ‘blocks’ Anti-Human ABCB1/MDR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human LNCaP prostate carcinoma (lanes 1 and 4), human HeLa cervix carcinoma (lanes 2 and 5) and human HepG2 liver carcinoma (lanes 3 and 6) cell line lysates:1-3. Anti-Human ABCB1/MDR1 Antibody (#AMT-021), (1:200).
4-6. Anti-Human ABCB1/MDR1 Antibody, preincubated with Human ABCB1/MDR1 Blocking Peptide (#BLP-MT021).
Properties
Sequence
- (C)RGSQAQDRKLSTKE, corresponding to amino acid residues 673-686 of human multidrug resistance protein 1 (Accession P08183).
Accession (Uniprot) Number P08183
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
