Human C5aR2 (extracellular) Blocking Peptide (#BLP-AR033) is the original antigen used for immunization during Anti-Human C5aR2 (extracellular) Antibody (#AAR-033) generation. The blocking peptide binds and ‘blocks’ Anti-Human C5aR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human MEG-O1 chronic myelogenous leukemia cell lines lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4):
- (C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2 (Accession Q9P296).