Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human CCR2 (extracellular) Blocking Peptide (#BLP-CR022) is the original antigen used for immunization during Anti-Human CCR2 (extracellular) Antibody (#ACR-022) generation. The blocking peptide binds and ‘blocks’ Anti-Human CCR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human acute monocytic leukemia THP-1 (lanes 1 and 3) and human promyelocytic leukemia HL-60 (lanes 2 and 4) cell line lysates:1, 2. Anti-Human CCR2 (extracellular) Antibody (#ACR-022), (1:1500).
3, 4. Anti-Human CCR2 (extracellular) Antibody, preincubated with Human CCR2 (extracellular) Blocking Peptide (#BLP-CR022).
Properties
Sequence
- RNTNESGEEVTTFFD(C), corresponding to amino acid residues 11-25 of human CCR2 (Accession P41597).
Accession (Uniprot) Number P41597
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
