Human CXCR2 (extracellular) Blocking Peptide (#BLP-CR012) is the original antigen used for immunization during Anti-Human CXCR2 (extracellular) Antibody (#ACR-012) generation. The blocking peptide binds and ‘blocks’ Anti-Human CXCR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human Jurkat acute T cell leukemia (lanes 1 and 4), human SH-SY5Y brain neuroblastoma (lanes 2 and 5) and human THP-1acute monocytic leukemia (lanes 3 and 6) lysates:
- (C)EDFNMESDSFEDFWKGED, corresponding to amino acid residues 2-19 of human CXCR2 (Accession P25025).