Human FPR1 (extracellular) Blocking Peptide (#BLP-FR001) is the original antigen used for immunization during Anti-Human FPR1 (extracellular) Antibody (#AFR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Human FPR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 5), human THP-1 acute monocytic leukemia (lanes 2 and 6), human T-84 colorectal carcinoma (lanes 3 and 7) and human U-87 MG glioblastoma (lanes 4 and 8) cell lysates:
- (C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1 (Accession P21462).