Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) is the original antigen used for immunization during Anti-Human GPR55 (extracellular) Antibody (#ACR-061) generation. The blocking peptide binds and ‘blocks’ Anti-Human GPR55 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human NK-92MI natural killer cell (lanes 1 and 3) (1:500) and human acute monocytic leukemia THP-1 cell (lanes 2 and 4) (1:200) lysates:
- Western blot analysis of human ovary adenocarcinoma OVCAR-3 cell (lanes 5 and 7) and human pancreatic carcinoma PANC-1 cell (lanes 6 and 8) lysates:
- Western blot analysis of human HMC3 microglia cell (lanes 9 and 11) (1:500) and human chronic myelogenous leukemia MEG-O1 cell (lanes 10 and 12) (1:200) lysates:
- Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55 (Accession Q9Y2T6).