Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human Orai1 (extracellular) Blocking Peptide (#BLP-CC060) is the original antigen used for immunization during Anti-Human Orai1 (extracellular) Antibody (#ACC-060) generation. The blocking peptide binds and ‘blocks’ Anti-Human Orai1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 2) and human HL-60 acute promyelocytic leukemia cell line lysate (lanes 3 and 4):1,3. Anti-Human Orai1 (extracellular) Antibody (#ACC-060), (1:200).
2,4. Anti-Human Orai1 (extracellular) Antibody, preincubated with Human Orai1 (extracellular) Blocking Peptide (#BLP-CC060).
Properties
Sequence
- (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31).
Accession (Uniprot) Number Q96D31
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
