Human Orai1 (extracellular) Blocking Peptide (#BLP-CC060) is the original antigen used for immunization during Anti-Human Orai1 (extracellular) Antibody (#ACC-060) generation. The blocking peptide binds and ‘blocks’ Anti-Human Orai1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 2) and human HL-60 acute promyelocytic leukemia cell line lysate (lanes 3 and 4):
- (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31).