Overview
Type: Synthetic peptide
Form: Lyophilized powder
IGF1R (extracellular) Blocking Peptide (#BLP-NT045) is the original antigen used for immunization during Anti-IGF1R (extracellular) Antibody (#ANT-045) generation. The blocking peptide binds and ‘blocks’ Anti-IGF1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1, 2. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
3, 4. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
4-6. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
Expression of IGF1R in rat cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. IGF1R staining (green) in the parietal cortex is detected in neurons of the pyramidal layer (arrows). B. IGF1R immunoreactivity (green) in the hippocampal CA1 region is observed neurons of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RQEYRKYGGAKLNR, corresponding to amino acid residues 883-896 of rat IGF1R (Accession P24062).
Accession (Uniprot) Number P24062
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
