IGF1R (extracellular) Blocking Peptide (#BLP-NT045) is the original antigen used for immunization during Anti-IGF1R (extracellular) Antibody (#ANT-045) generation. The blocking peptide binds and ‘blocks’ Anti-IGF1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
- Expression of IGF1R in rat cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. IGF1R staining (green) in the parietal cortex is detected in neurons of the pyramidal layer (arrows). B. IGF1R immunoreactivity (green) in the hippocampal CA1 region is observed neurons of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RQEYRKYGGAKLNR, corresponding to amino acid residues 883-896 of rat IGF1R (Accession P24062).