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Ionomycin calcium salt

A Potent and Selective Ca2+ Ionophore
Cat #: I-700
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Streptomyces conglobatus.
    Source Natural
    MW: 747.08
    Purity: >99% (HPLC)
    Effective concentration 100 nM - 2 µM.
    Structure
    Chemical name (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Tri hydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt.
    Molecular formula C41H70O9Ca.
    CAS No.: 56092-82-1.
    Activity Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria1,2. In mammalian cells, Ionomycin acts as a selective Ca2+ ionophore3. In addition, Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells as well as apoptosis in various cell lines4-6.
    References-Activity
    1. Liu, W.C. et al. (1978) J. Antibiot. (Tokyo) 31, 815.
    2. Liu, C. et al. (1978) J. Biol. Chem253, 5892.
    3. Kauffman, R.F. (1980) J. Biol. Chem255, 2735.
    4. Chatila, T. et al. (1989) J. Immunol. 143, 41283.
    5. Takei, N. et al. (1994) Brain Res652, 65.
    6. Aagaard-Tillery, K.M. and Jelinek D.F. (1995) J. Immunol. 155, 3297.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two week at 4°C or three months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs Ionomycin calcium salt induces cytosolic Ca2+ increase.
      Alomone Labs Ionomycin calcium salt induces cytosolic Ca2+ increase.
      Ca2+ traces from Fluo-3 AM loaded C6 glioma cells treated with 1 µM Ionomycin calcium salt (#I-700).
    References - Scientific background
    1. Liu, W.Cet al. (1978) J. Antibiot. (Tokyo) 31, 815.
    2. Liu, C. et al. (1978) J. Biol. Chem253, 5892.
    3. Kauffman, R.F. (1980) J. Biol. Chem255, 2735.
    4. Morgan, A.J. and Jacob, R. (1994) Biochem. J300, 665.
    5. Chatila, T. et al. (1989) J. Immunol. 143, 41283.
    6. Takei, N. et al. (1994) Brain Res652, 65.
    7. Aagaard-Tillery, K.M. and Jelinek D.F. (1995) J. Immunol. 155, 3297.
    Scientific background

    Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria produced by Streptomyces conglobatus. In mammalian cells, ionomycin acts as a potent and selective Ca2+ ionophore, more effective than A23187. The Ca2+ influx by ionomycin is caused by direct stimulation of store-regulated cation entry and not by direct action at the plasma membrane.1-4

    Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells and apoptosis in various cells lines.5-7

    Image & Title Ionomycin calcium salt
    Alomone Labs Ionomycin calcium salt increases [Ca2+]i in mouse sperm.Mouse sperm were incubated under capacitating conditions (CAP) and treated with soluble zona pellucida (sZP), which caused an increase in [Ca2+]i. Administration of Ionomycin calcium salt (#I-700) caused an additional increase in [Ca2+]i.Adapted from Wertheimer, E. et al. (2013) J. Biol. Chem. 288, 35307. with permission of The American Society for Biochemistry and Molecular Biochemistry.
    Last update: 16/08/2020

    Ionomycin calcium salt (#I-700) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Electrophysiology
    1. Human beta cells.
      Vierra, N.C. et al. 92017) Sci. Signal. 10, eaan2883.
    Product citations
    1. Kim, S.H. et al. (2013) J. Neurosci. 33, 8937.
    2. Wertheimer, E. et al. (2013) J. Biol. Chem. 288, 35307.
    3. Zamora, R. et al. (2012) J. Biol. Chem. 287, 31003.
    4. Martínez-López, P. et al. (2011) J. Cell. Physiol. 226, 1620.
    5. De Blas, G.A. et al. (2009) PLoS ONE 4, e6095.
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