KCC2/SLC12A5 Blocking Peptide (#BLP-KT005) is the original antigen used for immunization during Anti-KCC2 (SLC12A5) Antibody (#AKT-005) generation. The blocking peptide binds and ‘blocks’ Anti-KCC2/SLC12A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Western blot analysis of mouse brain lysates:
- Expression of KCC2 in rat cerebellum and hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. KCC2 immunoreactivity in the cerebellum (green) is detected in the granule layer (GL) and molecular layer (MOL). B. KCC2 staining in the hippocampal dentate gyrus region (green) is observed in the outer molecular layer (OML) and the hilus (H). Cell nuclei are stained with DAPI (blue).
- (C)KNPANTRLRLNVPE, corresponding to amino acid residues 967-980 of rat KCC2 (Accession Q63633).