Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCC2/SLC12A5 Blocking Peptide (#BLP-KT005) is the original antigen used for immunization during Anti-KCC2 (SLC12A5) Antibody (#AKT-005) generation. The blocking peptide binds and ‘blocks’ Anti-KCC2/SLC12A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:500).
2. Anti-KCC2 (SLC12A5) Antibody, preincubated with KCC2/SLC12A5 Blocking Peptide (#BLP-KT005).
Western blot analysis of mouse brain lysates:1. Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:200).
2. Anti-KCC2 (SLC12A5) Antibody, preincubated with KCC2/SLC12A5 Blocking Peptide (#BLP-KT005).
Expression of KCC2 in rat cerebellum and hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. KCC2 immunoreactivity in the cerebellum (green) is detected in the granule layer (GL) and molecular layer (MOL). B. KCC2 staining in the hippocampal dentate gyrus region (green) is observed in the outer molecular layer (OML) and the hilus (H). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KNPANTRLRLNVPE, corresponding to amino acid residues 967-980 of rat KCC2 (Accession Q63633).
Accession (Uniprot) Number Q63633
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
