KCNF1/Kv5.1 Blocking Peptide (#BLP-PC134) is the original antigen used for immunization during Anti-KCNF1 (KV5.1) Antibody (#APC-134) generation. The blocking peptide binds and ‘blocks’ Anti-KCNF1/Kv5.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 4), human SHSY-5Y neuroblastoma cell line lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
- (C)K(S)VWKFLEKPESS, corresponding to amino acid residues 166-178 of mouse KV5.1 (Accession Q7TSH7).