KCNG1/Kv6.1 (extracellular) Blocking Peptide (#BLP-PC135) is the original antigen used for immunization during Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135) generation. The blocking peptide binds and ‘blocks’ Anti-KCNG1/Kv6.1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of KV6.1 potassium channel in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135), (1:200). A. KV6.1 is detected in rat cerebellum (red). Staining is apparent in Purkinje neurons (arrow) and in the molecular layer (Mol). B. Cell nuclei in the same section are stained with DAPI (blue). C. Merge of the two images.
- (C)RRKPSTGNSYLDK, corresponding to amino acid residues 325-337 of mouse KV6.1 (Accession A2BDX4).