KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122) is the original antigen used for immunization during Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK18/TRESK (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes:
- Western blot analysis of mouse brain membranes:
- Expression of KCNK18 in rat dorsal root gangliaImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining).
- (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18 (Accession Q6VV64).