Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNS1/Kv9.1 (extracellular) Blocking Peptide (#BLP-PC131) is the original antigen used for immunization during Anti-KCNS1 (KV9.1) (extracellular) Antibody (#APC-131) generation. The blocking peptide binds and ‘blocks’ Anti-KCNS1/Kv9.1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 4), and rat (lanes 2 and 5) brain membranes and human HL-60 promyelocytic leukemia cell lysates (lanes 3 and 6):1-3. Anti-KCNS1 (KV9.1) (extracellular) Antibody (#APC-131), (1:200).
4-6. Anti-KCNS1 (KV9.1) (extracellular) Antibody, preincubated with KCNS1/Kv9.1 (extracellular) Blocking Peptide (#BLP-PC131).
Properties
Sequence
- (C)EDVRDDPVLRRLE, corresponding to amino acid residues 233-245 of rat KCNS1 (Accession O88758).
Accession (Uniprot) Number O88758
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
