Kidins220 Blocking Peptide (#BLP-PZ017) is the original antigen used for immunization during Anti-Kidins220 Antibody (#APZ-017) generation. The blocking peptide binds and ‘blocks’ Anti-Kidins220 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4):
- Expression of Kidins220 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Kidins220 Antibody (#APZ-017), (1:1000), followed by goat-anti-rabbit-AlexaFluor-488. A. Kidins220 staining (green) in hippocampal sections is seen in neurons in the pyramidal layer (Pyr) and stratum oriens (OR) (arrows). B. Staining in cerebellum sections shows labeling (green) in neurons in the Purkinje cell layer (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)PRHSNLSWQSQTRR, corresponding to amino acid residues 1335-1348 of rat Kidins220 (Accession Q9EQG6).