Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026) is the original antigen used for immunization during Anti-Kir2.1/KCNJ2 Antibody (#APC-026) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.1/KCNJ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart (lanes 1 and 3) and rat brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.1/KCNJ2 Antibody (#APC-026), (1:200).
3,4. Anti-Kir2.1/KCNJ2 Antibody, preincubated with Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026).- Rat brain sections.
Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Guinea pig Anti-Kir2.1/KCNJ2 Antibody (#APC-026-GP), (1:500).
3-4. Guinea pig Anti-Kir2.1/KCNJ2 Antibody, preincubated with Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026).
Western blot analysis of human U-87 MG glioblastoma cell lysate:1. Guinea pig Anti-Kir2.1/KCNJ2 Antibody (#APC-026-GP), (1:500).
2. Guinea pig Anti-Kir2.1/KCNJ2 Antibody, preincubated with Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026).
Properties
Sequence
- (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049).
Accession (Uniprot) Number P48049
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
