Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026) is the original antigen used for immunization during Anti-Kir2.1/KCNJ2 Antibody (#APC-026) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.1/KCNJ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart (lanes 1 and 3) and rat brain (lanes 2 and 4) membranes:
- Rat brain sections.
- Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human U-87 MG glioblastoma cell lysate:
- (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049).