Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125) is the original antigen used for immunization during Anti-Kir7.1 (extracellular) Antibody (#APC-125) generation. The blocking peptide binds and ‘blocks’ Anti-Kir7.1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse kidney lysates (lanes 3 and 6):
- Expression of Kir7.1 in rat kidneyImmunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:100). Staining is present in both distal (DT) and proximal (PT) tubules and in the collecting ducts (CD) in the renal cortex. Note that staining is absent both in glomeruli (Gl) and blood vessels (arrow). Hematoxilin is used as the counterstain.
- (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat KCNJ13 (Accession O70617).