Every lot is tried & tested in a relevant biological assay.
Our Bioassay
- Chessell, I.P. et al. (1998) Br. J. Pharmacol.124, 1314.
- Alomone Labs KN-62 inhibits human P2X7 receptors expressed in HEK-293 cells.Dose-response curve of hP2X7 inhibition by KN-62 (#K-120). Cells were loaded with Fluo-8 NW dye, incubated with increasing concentrations of KN-62, and stimulated with 80 µM BzATP. Changes in intracellular Ca2+ following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA™. IC50 was calculated at 2.6 µM.
- Chessell, I.P. et al. (1998) Br. J. Pharmacol.124, 1314.
- Min, J.W. et al. (2017) Oncotarget 8, 25513.
- Bhattacharya, A. et al. (2013) Br. J. Pharmacol. 170, 624.
KN-62 is a potent and non-competitive antagonist of purinergic P2X7 receptors. In addition, KN-62 acts as a selective cell permeable inhibitor of Ca2+/calmodulin-dependent kinase type II (CaM kinase II)1. KN-62 inhibits Bz-ATP-induced currents in HEK–human P2X7 cells and CaM kinase II with IC50 value of 25 nM and 1 µM respectively1.
CaM kinase II is very important to synaptogenesis and plasticity during brain development2. The P2X receptors are a family of ion channels that gated by ATP, a ligand that mediates wide critical physiologic functions. P2X7 receptor is responsible for mediating the release of proinflammatory cytokines in inflammatory/immune conditions and pain3.
KN-62 (#K-120) is a highly pure, synthetic, and biologically active compound.
Applications
Citations
Specifications
Scientific Background
Related Products
- Anti-P2X7 Receptor Antibody (#APR-004)
- Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO)
- Anti-P2X7 Receptor (extracellular) Antibody (#APR-008)
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG)
- Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F)
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR)
- HMA (#H-130)
- Chelerythrine chloride (#C-400)
- AZ11645373 (#A-395)
- AZ 10606120 dihydrochloride (#A-405)
- AACBA hydrochloride (#A-410)
- A-804598 (#A-415)
- A-740003 (#A-420)
- AZD-9056 hydrochloride (#A-425)
- A-839977 (#A-470)
- GW 791343 hydrochloride (#G-115)
- JNJ-47965567 (#J-115)
- JNJ-54166060 (#J-125)
- ZINC58368839 (#Z-135)
- ZINC9315614 (#Z-145)
- CAY10593 (VU0155069) (#C-385)
- Garcinolic acid (#G-155)
- BzATP triethylammonium salt (#A-385)
- P2X7 Receptor Basic Research Pack (#ESB-710)
- P2X7 Receptor Premium Research Pack (#ESP-710)
- P2X7 Receptor Deluxe Research Pack (#ESD-710)
Resources
Need Help With This Product?
Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.
Call us at
+972 2587 2202 or Email Us