Kv2.1 Blocking Peptide (#BLP-PC012) is the original antigen used for immunization during Anti-KV2.1 Antibody (#APC-012) generation. The blocking peptide binds and ‘blocks’ Anti-Kv2.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:1. Anti-KV2.1 Antibody (#APC-012), (1:500).
2. Anti-KV2.1 Antibody, preincubated with KV2.1 Blocking Peptide (BLP-PC012).
- Expression of KV2.1 channel in rat pancreasImmunohistochemical staining of rat paraffin embedded pancreas sections using Anti-KV2.1 Antibody (#APC-012), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. Endocrine (Islets of Langerhans) and exocrine areas of pancreas section are shown. Strong and highly specific KV2.1 staining is evident only in endocrine cells (red). Hoechst 33342 is used as the counterstain (blue).
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of KV2.1 channel in rat hippocampusImmunohistochemical staining of rat hippocampal CA1 region using Guinea pig Anti-KV2.1 Antibody (#APC-012-GP), (1:400). A. KV2.1 staining (green) appears in the pyramidal layer (P) and in stratum oriens (SO) interneurons (horizontal arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.
- (C)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1 (Accession P15387).