Overview
Type: Synthetic peptide
Form: Lyophilized Powder
KV8.1 (KCNV1) Blocking Peptide (#BLP-PC139) is the original antigen used for immunization during Anti-KV8.1 (KCNV1) Antibody (#APC-139) generation. The blocking peptide binds and ‘blocks’ Anti-KV8.1 (KCNV1) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-KV8.1 (KCNV1) Antibody (#APC-139), (1:200).
3-4. Anti-KV8.1 (KCNV1) Antibody, preincubated with KV8.1 (KCNV1) Blocking Peptide (BLP-PC139).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-KV8.1 (KCNV1) Antibody (#APC-139), (1:500).
2. Anti-KV8.1 (KCNV1) Antibody, preincubated with KV8.1 (KCNV1) Blocking Peptide (BLP-PC139).
Properties
Sequence
- Peptide (C)KKDTDDQESQHESE, corresponding to amino acid residues 171 - 184 of rat KCNV1 (Accession P97557).
Accession (Uniprot) Number P97557
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
