Overview
Type: Synthetic peptide
Form: Lyophilized powder
LAT1/SLC7A5 Blocking Peptide (#BLP-NT105) is the original antigen used for immunization during Anti-LAT1 (SLC7A5) Antibody (#ANT-105) generation. The blocking peptide binds and ‘blocks’ Anti-LAT1/SLC7A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat placenta lysate (lanes 1 and 4), mouse testes lysate (lanes 2 and 5) and rat skeletal muscle membrane (lanes 3 and 6):1-3. Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200).
4-6. Anti-LAT1 (SLC7A5) Antibody, preincubated with LAT1/SLC7A5 Blocking Peptide (#BLP-NT105).
Expression of LAT1/SLC7A5 in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. LAT1 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with LAT1 (SLC7A5) Blocking Peptide (#BLP-NT105), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KDIGQGDASNLHQK, corresponding to amino acid residues 222-235 of rat LAT1 (Accession Q63016).
Accession (Uniprot) Number Q63016
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
