LETM1 Blocking Peptide (#BLP-NT183) is the original antigen used for immunization during Anti-LETM1 Antibody (#ANT-183) generation. The blocking peptide binds and ‘blocks’ Anti-LETM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human acute lymphoblastic leukemia (MOLT-4) (lanes 3 and 7) and human colorectal adenocarcinoma (HT-29) cell lysates:
- (C)KELSKTGDEKYIEE, corresponding to amino acid residues 587-600 of rat LETM1 (Accession Q5XIN6).