LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032) is the original antigen used for immunization during Anti-LINGO-1 (extracellular) Antibody (#ANT-032) generation. The blocking peptide binds and ‘blocks’ Anti-LINGO-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of LINGO-1 in rat brain and spinal cordImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:400), followed by donkey-anti-rabbit-Cy3. A. LINGO-1 staining (red) is detected in sections of rat substantia nigra, in nigral neurons (arrows). B. Staining of rat spinal cord shows LINGO-1 labeling in ventral horn neurons (arrows). Cell nuclei are stained with DAPI (blue).
- (C)HVRSYSPDWPHQPNK, corresponding to amino acid residues 507-521 of mouse LINGO-1 (Accession Q9D1T0).