Lynx1 (extracellular) Blocking Peptide (#BLP-NC021) is the original antigen used for immunization during Anti-Lynx1 (extracellular) Antibody (#ANC-021) generation. The blocking peptide binds and ‘blocks’ Anti-Lynx1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
- Expression of Lynx1 in rat dorsal raphe nucleus and substantia nigra pars compactaImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Lynx1 staining in the dorsal raphe nucleus (DRN), (green) is detected in cells of the medial DRN (MDRN, horizontal arrows) and in cells of the lateral DRN (LDRN), (vertical arrows). B. Lynx1 immunoreactivity in the substantia nigra pars compacta (SNC), (green) is observed in cells of the SNC (arrows). Cell nuclei are stained with DAPI (blue).
- CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1 (Accession P0DP60).