Overview
Type: Synthetic peptide
Form: Lyophilized powder
MAGI2/AIP1 Blocking Peptide (#BLP-PZ052) is the original antigen used for immunization during Anti-MAGI2/AIP1 Antibody (#APZ-052) generation. The blocking peptide binds and ‘blocks’ Anti-MAGI2/AIP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate:1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:400).
2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
Western blot analysis of rat brain membranes:1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:400).
2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-MAGI2/AIP1 Antibody (#APZ-052), (1:200).
2. Anti-MAGI2/AIP1 Antibody, preincubated with MAGI2/AIP1 Blocking Peptide (#BLP-PZ052).
Properties
Sequence
- (C)ENSYRSEVKARQDVK, corresponding to amino acid residues 1066 - 1080 of mouse MAGI2 (Accession Q9WVQ1).
Accession (Uniprot) Number Q9WVQ1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
