MANF/ARMET Blocking Peptide (#BLP-NT028) is the original antigen used for immunization during Anti-MANF/ARMET Antibody (#ANT-028) generation. The blocking peptide binds and ‘blocks’ Anti-MANF/ARMET primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human recombinant MANF protein (lanes 3 and 6):
- Expression of MANF in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MANF/ARMET Antibody (#ANT-028), (1:600), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Sections derived from parietal cortex of a rat at postnatal day 22 reveal MANF staining (green) in nuclei and cytoplasm of several cells (arrows) in deep layers of the cortex. Nuclei are stained with DAPI (blue).
- (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF (Accession P55145).