MC2 Receptor (extracellular) Blocking Peptide (#BLP-MR022) is the original antigen used for immunization during Anti-MC2 Receptor (extracellular) Antibody (#AMR-022) generation. The blocking peptide binds and ‘blocks’ Anti-MC2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat adrenal lysate:
- Expression of MC2R in rat adrenal glandImmunohistochemical staining of paraffin embedded rat adrenal cortex section using Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:100). Shown are the capsule (C), the zona glomerulosa (ZG) and the zona fasciculata (ZF) of the adrenal cortex. Note that the staining (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule. Color reaction was obtained with DAB. H&E is used as the counterstain.
- (C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R (Accession Q01718).