MC3 Receptor (extracellular) Blocking Peptide (#BLP-MR023) is the original antigen used for immunization during Anti-MC3 Receptor (extracellular) Antibody (#AMR-023) generation. The blocking peptide binds and ‘blocks’ Anti-MC3 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Expression of MC3R in rat brainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:100). Expression (green) appears in the hypothalamic periventricular region (horizontal arrow), in some nerve cells (vertical arrow), and in the neuropil (asterisk). Hoechst 33342 is used as the counterstain (blue).
- (C)NSDSLTLEDQFIQHMD, corresponding to amino acid residues 102-117 of rat MC3R (Accession P32244).