Overview
Type: Synthetic peptide
Form: Lyophilized powder
MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024) is the original antigen used for immunization during Anti-MC4 Receptor (extracellular) Antibody (#AMR-024) generation. The blocking peptide binds and ‘blocks’ Anti-MC4 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024).
Western blot analysis of mouse brain lysates:1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024).
Expression of MC4R in mouse brainImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:100). MC4R (green) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245).
Accession (Uniprot) Number P32245
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
