MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024) is the original antigen used for immunization during Anti-MC4 Receptor (extracellular) Antibody (#AMR-024) generation. The blocking peptide binds and ‘blocks’ Anti-MC4 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysates:
- Western blot analysis of mouse brain lysates:
- Expression of MC4R in mouse brainImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:100). MC4R (green) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).
- (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245).