MCT1/SLC16A1 (extracellular) Blocking Peptide (#BLP-MT011) is the original antigen used for immunization during Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011) generation. The blocking peptide binds and ‘blocks’ Anti-MCT1/SLC16A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of rat skeletal muscle lysate:
- Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigment epithelium cell line lysate (lanes 2 and 4):
- Expression of monocarboxylate transporter 1 in mouse hippocampusImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011), (1:200), followed by goat-anti-rabbit-Cy3. A. MCT1 staining (red) in CA1 hippocampal region, is detected in the pyramidal layer (P) and in small cell outlines (arrows). B. GFAP staining (green) is observed in astrocyte outlines (arrows). C. Merge of the two images demonstrates co-localization of MCT1 and GFAP in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
- (C)GRLNDMYGDYKYT, corresponding to amino acid residues 403-415 of rat MCT1 (Accession P53987).