Melatonin Receptor 1A/MTNR1A Blocking Peptide (#BLP-MR031) is the original antigen used for immunization during Anti-Melatonin Receptor 1A (MTNR1A) Antibody (#AMR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Melatonin Receptor 1A/MTNR1A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:
- Expression of Melatonin receptor type 1A in rat supra-chiasmatic nucleusImmunohistochemical staining of rat supra-chiasmatic nucleus (SCN) with Anti-Melatonin Receptor 1A (MTNR1A) Antibody (#AMR-031). A. MT1 staining is restricted to the SCN (red). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
- (C)RVKPDNKPKLKPQD, corresponding to amino acid residues 223-236 of mouse MTNR1A (Accession Q61184).