Overview
Type: Synthetic peptide
Form: Lyophilized powder
Melatonin Receptor 1B/MTNR1B Blocking Peptide (#BLP-MR032) is the original antigen used for immunization during Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032) generation. The blocking peptide binds and ‘blocks’ Anti-Melatonin Receptor 1B/MTNR1B primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:200).
3,4. Anti-Melatonin Receptor 1B (MTNR1B) Antibody, preincubated with Melatonin Receptor 1B/MTNR1B Blocking Peptide (#BLP-MR032).
Multiplex staining of Melatonin receptor type 1B and NaV1.2 in rat paraventricular nucleusImmunohistochemical staining of perfusion-fixed frozen rat paraventricular nucleus sections using Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:600) and Guinea pig Anti-SCN2A (NaV1.2) Antibody (#ASC-002-GP), (1:2000). A. MT2 staining (red) (arrows). B. The same section labeled for NaV1.2 (green). C. Merge of A and B demonstrates partial co-localization of MT2 and NaV1.2 in the paraventricular nucleus (PVN). For orientation, note location with respect to the third ventricle (3rd V). Cell nuclei were stained with DAPI (blue).
Multiplex staining of Melatonin receptor type 1B and Angiotensin II receptor type 2 in rat brainImmunohistochemical staining of perfusion-fixed frozen brain sections using Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:600) and Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#AAR-012-AG), (1:100). A. MT2 staining (red) (arrows). B. The same section labeled for Angiotensin II receptor type-2 (green). C. Merge of the two images suggests considerable co-localization in the paraventricular nucleus (arrows). For orientation, note localization with respect to 3rd ventricle (3rd V).
Expression of Melatonin receptor type 1B in rat supraoptic nucleusImmunohistochemical staining of perfusion-fixed frozen brain sections using Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:600), (red). MT2 is expressed discretely in the supraoptic nucleus (SON), adjacent to the optric tract (OT). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)RKAKATRKLRLRPSD, corresponding to amino acid residues 232-246 of mouse MTNR1B (Accession Q8CIQ6).
Accession (Uniprot) Number Q8CIQ6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
