Overview
Type: Synthetic peptide
Form: Lyophilized powder
mGluR2 (extracellular) Blocking Peptide (#BLP-GC011) is the original antigen used for immunization during Anti-mGluR2 (extracellular) Antibody (#AGC-011) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes:1,2. Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:400).
3,4. Anti-mGluR2 (extracellular) Antibody, preincubated with mGluR2 (extracellular) Blocking Peptide (#BLP-GC011).
Western blot analysis of mouse brain membranes:1. Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:200).
2. Anti-mGluR2 (extracellular) Antibody, preincubated with mGluR2 (extracellular) Blocking Peptide (#BLP-GC011).
Expression of mGluR2 in rat brainImmunohistochemical staining of perfusion-fixed brain frozen sections using Anti-mGluR2 (extracellular) Antibody (#AGC-011). A. mGluR2 (green) is visualized in the corpus callosum (CC) and hippocampal stratum oriens (OR). B. Glial fibrillary acidic protein (GFAP) (red), a marker of astrocytes. C. Merge of the two images demonstrates expression of mGluR2 in astrocytes. DAPI is used as the nuclear counterstain (blue).
Properties
Sequence
- SLSRGADGSRHIC, corresponding to amino acids 109-121 of rat mGluR2 (Accession P31421).
Accession (Uniprot) Number P31421
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
