mGluR8 (extracellular) Blocking Peptide (#BLP-GC028) is the original antigen used for immunization during Anti-mGluR8 (extracellular) Antibody (#AGC-028) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
- Expression of mGluR8 in rat globus pallidusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.
- (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8 (Accession P70579).