Every lot is tried & tested in a relevant biological assay.
Centrifuge all products before handling (10000 x g 5 min).
It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.
- Alomone Labs ML-301 activates human NTR1 receptors expressed in CHO-K1 cells.Dose-response curve of hNTSR1 activation by ML-301 (#M-315), normalized to maximum effect of neurotensin. Cells were loaded with Fluo-8 dye and stimulated with increasing concentrations of ML-301. Changes in intracellular Ca2+ following agonist application were detected as changes in maximum relative fluorescence using FLIPRTETRA™.
ML-301 is a fully selective imidazole-based agonist of neurotensin receptor 1 (NTR1). It stimulates NTR1-mediated calcium mobilization with an EC50 value of 289 nM1. The carboxylic acid moiety increases the compound’s solubility1.
To date, four neurotensin receptors have been cloned from human: NTR1, NTR2, NTR3 and NTR4 which belong to the super family of G-protein coupled receptors (GPCRs). NTR1 mediates most of the known neurotensin ligand effects and is a potential therapeutic target2.
ML-301 (#M-315) is a highly pure, synthetic, and biologically active compound.