Mouse proBDNF Deluxe Research Pack

All You Need for proBDNF Research
  • Lyophilized Powder
  • Antigen Incl.
  • Fluorescent Label
  • Bioassay Tested
  • QC Tested
  • Shipped at Room Temp.
  • Live Cell Imaging
  • Immuno-Colocalization
  • 100% Net Peptide
  • Sterile & Endotoxin Free
Cat #: ESD-602
Sizes: 12 Vials
Last update: 16/04/2018

Alomone Labs is pleased to offer the Mouse proBDNF Deluxe Research Pack (#ESD-602). The Research Pack contains all you need for mouse proBDNF research: Antibodies to proBDNF and its receptor, and recombinant proteins all in one economical package!

For research purposes only, not for human use
Compounds
Product NameCat #Size
Anti-p75NTR (extracellular) Antibody
ANT-007 1 x 0.2 ml
Anti-p75NTR (extracellular)-ATTO-550 Antibody
ANT-007-AO 1 x 50 µl
Anti-proBDNF Antibody
ANT-006 1 x 0.2 ml
Anti-proBDNF-ATTO-488 Antibody
ANT-006-AG 1 x 50 µl
Guinea pig Anti-proBDNF Antibody
AGP-032 1 x 0.2 ml
mouse proBDNF
B-240 1 x 25 µg
mouse proBDNF (cleavage resistant)
B-243 1 x 25 µg
Note A guinea pig polyclonal antibody (#AGP-032) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
Scientific Background
Scientific Background

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.

The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the pro domain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP) a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer’s disease.

Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

References
  1. Lee R. et al. (2001) Science 294,1945.
  2. Egan, M.F. et al. (2003) Cell 112, 257.
  3. Pang, P.T. et al. (2004) Science 306, 487.
  4. Michalski, B. et al. (2003) Mol. Brain Res. 111, 148.
Related Products