MRGPRE Blocking Peptide (#BLP-MR062) is the original antigen used for immunization during Anti-MRGPRE Antibody (#AMR-062) generation. The blocking peptide binds and ‘blocks’ Anti-MRGPRE primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:
- Western blot analysis of human U-87 MG glioblastoma:
- Expression of MRGE in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE (Accession Q7TN40).