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N-Formyl-Met-Leu-Phe

FMLP, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, N-formyl-MLF

A Potent Agonist of FPR1 G-Protein Coupled Receptor

Cat #: GPF-100
Alternative Name FMLP, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, N-formyl-MLF
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 437.6 Da
    Purity: >98% (HPLC)
    Effective concentration 1 nM - 10 μM.
    Sequence MLF.
    Modifications Met1 - N-formyl-Met.
    Molecular formula C21H31N3O5S.
    CAS No.: 59880-97-6
    Activity N-Formyl-Met-Leu-Phe is a selective and potent agonist of the formyl peptide receptor (FPR1)1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs N-Formyl-Met-Leu-Phe activates Ca2+ transients in differentiated HL-60 cells.
      Alomone Labs N-Formyl-Met-Leu-Phe activates Ca2+ transients in differentiated HL-60 cells.
      Cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+ were detected via changes in Fluo-3 emission following application (indicated by arrow) of 1 µM N-Formyl-Met-Leu-Phe (#GPF-100), (green) compared to control (black, saline perfusion).
    References - Scientific background
    1. Ye, R.D. et al. (2009) Pharmacol. Rev. 61, 119.
    2. Le, Y. et al. (2002) Trends Immunol. 23, 541.
    3. Showell, H.J. et al. (1976) J. Exp. Med 143, 1154.
    4. Krump, E. et al. (1997) J. Biol. Chem. 272, 937.
    5. Tonnesen, M.G. et al. (1984) J. Clin. Invest. 74, 1581.
    Scientific background

    Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.

    N-Formyl-Met-Leu-Phe is a selective and potent agonist of the Formyl peptide receptor (FPR1)3.

    In human polymorphonuclear leukocytes N-formyl-met-leu-phe activates p38 by a process involving phosphatidylinositol 3-kinase, protein kinase C, and calcium4. N-Formyl-Met-Leu-Phe increased in a dose-dependent manner (0.1 nM – 1 µM) the adherence of neutrophils to vascular endothelial cells which is the initial event in the migration of neutrophils through blood vessel walls to tissue sites of inflammation5.

    Target

    FPR1 receptor

    Net Peptide Content: 100%
    Lyophilized Powder
    Last update: 11/04/2021

    N-Formyl-Met-Leu-Phe (#GPF-100) is a highly pure, synthetic, and biologically active peptide.

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    For research purposes only, not for human use

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