Overview
Type: Synthetic peptide
Form: Lyophilized powder
Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 6/NHE-6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4):1,2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
3,4. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Western blot analysis of mouse brain lysate:1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Expression of NHE-6 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NHE-6 staining (green) in hippocampal dentate gyrus region, appears in neuronal soma outlines in the hilus (horizontal arrows) and the outer granule layer (vertical arrows). Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- (C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6 (Accession Q92581).
Accession (Uniprot) Number Q92581
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
