Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 6/NHE-6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4):
- Western blot analysis of mouse brain lysate:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of NHE-6 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NHE-6 staining (green) in hippocampal dentate gyrus region, appears in neuronal soma outlines in the hilus (horizontal arrows) and the outer granule layer (vertical arrows). Cell nuclei were stained with DAPI (blue).
- (C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6 (Accession Q92581).