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Certificate of Analysis

NaV1.2 Channel Premium Research Pack

All You Need for NaV1.2 Channel Research

Cat #: ESP-002
Size: 7 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Homology Mouse, Human - identical.
NaV1.3 – 12 out of 19 amino acid residues identical

Guinea pig Anti-SCN2A (NaV1.2) Antibody Cat #: AGP-026

Size: 0.2 ml Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.

Pharmacological Reagents

α-Pompilidotoxin Cat #: P-170

Size: 10 mg MW: 1529 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 µM.
Activity
α-Pompilidotoxin slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Ceratotoxin-1 Cat #: STC-680

Size: 0.1 mg MW: 4044 Da Purity: >99% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-900 nM.
Activity
Ceratotoxin-1 is a NaV channel blocker1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Hainantoxin-IV Cat #: STH-130

Size: 0.1 mg MW: 3987 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
20-250 nM.
Activity
Neural TTX-sensitive voltage-gated Na+ channel inhibitor1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

QX-314 chloride Cat #: Q-150

Size: 100 mg Source: Synthetic MW: 298.85 Purity: >99% (HPLC)
Effective concentration
1-50 mM.
Activity
QX-314 chloride is an intracellular blocker of NaV channels1-4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for four years.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or six months at -20°C.
Protect from light. Hygroscopic.

ATX-II Cat #: STA-700

Size: 0.1 mg MW: 4935 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A guinea pig polyclonal antibody (#AGP-026) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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