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Anti-SCN2A (NaV1.2)-ATTO-594 Antibody

BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
    Cat #: ASC-002-AR
    Alternative Name BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
    • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
    • Anti-SCN2A (NaV1.2)-ATTO-594 Antibody
    Accession (Uniprot) Number P04775
    Gene ID 24766
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse – identical.
    RRID AB_2341065.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASC-002), and immunohistochemistry (labeled antibody).
    Applications: if, ih
    May also work in: ic*
    Immunohistochemistry
    • Anti-SCN2A (NaV1.2)-ATTO-594 Antibody
      Expression of NaV1.2 in rat hippocampus
      Immunohistochemical staining of rat hippocampus using Anti-SCN2A (NaV1.2)-ATTO-594 Antibody (#ASC-002-AR). A. Staining of NaV1.2 (red) appears in the stratum radiatum (CA3-SR) apical dendrites (arrow) of pyramidal neurons (Pyr). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
    8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

    NaV1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the NaV1.2 channel have been identified in different types of epilepsy.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderNegative Control Antigen Included
    Last update: 24/01/2020

    Anti-SCN2A (NaV1.2) Antibody (#ASC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.2 from rat, human, and mouse samples.

    Anti-SCN2A (NaV1.2)-ATTO-594 Antibody (#ASC-002-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-SCN2A (NaV1.2)-ATTO-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse brain sections with #ASC-002. Tested in SCN2A-/- mice.
      Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.

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