Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nav1.6/SCN8A Blocking Peptide (#BLP-SC009) is the original antigen used for immunization during Anti-NaV1.6 (SCN8A) Antibody (#ASC-009) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.6/SCN8A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-NaV1.6 (SCN8A) Antibody (#ASC-009), (1:200).
2. Anti-NaV1.6 (SCN8A) Antibody, preincubated with Nav1.6/SCN8A Blocking Peptide (#BLP-SC009).
Expression of NaV1.6 in mouse hippocampusImmunohistochemical staining of mouse hippocampus using Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). A. NaV1.6 (green) is robustly expressed in the CA1 pyramidal layer (white arrows). B. Staining with mouse anti-parvalbumin (red), a marker of interneurons. C. Merged image of panels A and B reveals that NaV1.6 appears in some interneurons (arrow) but is not restricted to interneurons. DAPI is used as the counterstain.- Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
Properties
Sequence
- CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6 (Accession O88420).
Accession (Uniprot) Number O88420
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
