Cat #: BLP-NX013
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-NCX3 (SLC8A3) Antibody (#ANX-013). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):1-3. Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:200).
4-6. Anti-NCX3 (SLC8A3) Antibody, preincubated with NCX3/SLC8A3 Blocking Peptide (#BLP-NX013).
- Expression of NCX3 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:100). A. NCX3 staining (green) is expressed mostly in molecular layer (Mol) interneurons (arrow). B. The same section, stained with Parvalbumin (Red). C. Merge of A and B demonstrates that NCX3 appears to be expressed in several GABAergic neurons in the molecular layer of which Parvalbumin positive cells are only one sub-group (see arrow). DAPI counterstain (blue) displays the layout of cerebellar layers.
- (C)PLEGKEVDESRRE, corresponding to amino acid residues 303-315 of rat NCX3 (Accession P70549).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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