Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051) is the original antigen used for immunization during Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-1/PVRL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell lysates:
- Expression of Nectin-1 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:100). A. Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
- (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 of human Nectin-1 (Accession Q15223).