Overview
Type: Synthetic peptide
Form: Lyophilized powder
NETO2 (extracellular) Blocking Peptide (#BLP-GC046) is the original antigen used for immunization during Anti-NETO2 (extracellular) Antibody (#AGC-046) generation. The blocking peptide binds and ‘blocks’ Anti-NETO2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200).
3,4. Anti-NETO2 (extracellular) Antibody, preincubated with NETO2 (extracellular) Blocking Peptide (#BLP-GC046).
Western blot analysis of human SH-SY5Y neuroblastoma (lanes 1 and 4), human HT-29 colon adenocarcinoma (lanes 2 and 5) and human MCF-7 breast adenocarcinoma (lanes 3 and 6) cell line lysates:1-3. Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200).
4-6. Anti-NETO2 (extracellular) Antibody, preincubated with NETO2 (extracellular) Blocking Peptide (#BLP-GC046).
Expression of NETO2 in mouse and rat cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NETO2 immunoreactivity (green) in both mouse (A.) and rat (B.) cerebellum region is detected in the granule layer (G) and in “baskets” (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RSSQVEQEEKTKPG, corresponding to amino acid residues 189-202 of rat NETO2 (Accession C6K2K4).
Accession (Uniprot) Number C6K2K4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
