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Certificate of Analysis

  • Bioassay Tested

Neural Stem Cell Culturing Kit

A Neural Stem Cell Culturing Kit Economically Priced

Cat #: EK-430
Size: 7 Vials
Form: Lyophilized

Recombinant human BDNF protein Cat #: B-250

Size: 10 µg MW: 27 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
ED50 = 220 pM.
Activity
BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 µg/0.1 ml. BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human CNTF protein Cat #: C-240

Size: 25 µg MW: 22.9 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
EC50 = 1 nM.
Activity
CNTF is a potent neurotrophic factor that was originally characterized as a survival factor for chick ciliary neurons. CNTF supports growth and survival of DRG, motor and sympathetic neurons1-4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 μg/0.1 ml. BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human EGF protein Cat #: E-100

Size: 0.2 mg MW: 6.2 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
EC50 = 20 pg/ml.
Activity
EGF is a potent growth factor that stimulates both in vitro and in vivo the proliferation of various epidermal and epithelial cells1. Fibroblasts are also stimulated in vitro by EGF2,3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 μg/0.1 ml. BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human FGF-b protein Cat #: F-170

Size: 25 µg MW: 16.5 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
EC50 = ~17 pM.
Activity
FGF-basic is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal, endothelial, and smooth muscle cells, among many others. FGF-basic also induces neuronal and glial differentiation, survival and regeneration1-4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 µg/0.1 ml.
BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human GDNF protein Cat #: G-240

Size: 10 µg MW: 30.1 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Activity
GDNF enhances survival and differentiation of dopaminergic neurons. It is also a potent survival factor for motor neurons1-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 µg/0.1 ml.
Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human LIF protein Cat #: L-200

Size: 10 µg MW: 19.7 kDa Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
EC50 = ~10 pM.
Activity
LIF is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF regulates cholinergic, peptidergic and noradrenergic properties of sympathetic neurons and rescues sensory and motor neurons from death. LIF also promotes differentiation of O-2A astrocytes1-4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 µg/0.1 ml.
BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant rat IGF-I protein Cat #: I-200

Size: 25 µg MW: 7692 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0 ng/ml, corresponding to a specific activity of >500,000 units/mg.
rat IGF-I is fully biologically active when compared to standards. The ED50 shown in literature was calculated in two different methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less than 30 ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be less than 10 ng/ml.
Activity
IGF-I is a pleiotropic factor involved in multiple processes, so its actions are different depending on its concentration, the cell type, and the developmental stage of the animal1. IGF-I promotes proliferation of neural cells by interacting with the IGF-IR which may activate the PI3K/Akt or the MAP kinase pathways1.
Evidence indicates that IGF-I promotes cell survival by inhibiting apoptosis both in vivo and in vitro. IGF-I is also involved in the regulation of the migration of certain cell types1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 1 µg/0.1 ml. BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
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