Cat #: BLP-NR031
Type: Synthetic peptide
Form: Lyophilized powder
Neurexin 1α (extracellular) Blocking Peptide (#BLP-NR031) is the original antigen used for immunization during Anti-Neurexin 1α (extracellular) Antibody (#ANR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Neurexin 1α (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-Neurexin 1α (extracellular) Antibody (#ANR-031), (1:400).
3,4. Anti-Neurexin 1α (extracellular) Antibody, preincubated with Neurexin 1α (extracellular) Blocking Peptide (#BLP-NR031).
- (C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Nrxn1α (Accession Q63372).
Accession (Uniprot) Number Q63372
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.