Cat #: BLP-NR033
Type: Synthetic peptide
Form: Lyophilized powder
Neurexin 3α (extracellular) Blocking Peptide (#BLP-NR033) is the original antigen used for immunization during Anti-Neurexin 3α (extracellular) Antibody (#ANR-033) generation. The blocking peptide binds and ‘blocks’ Anti-Neurexin 3α (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), human brain neuroblastoma (SH-SY5Y) cells (lanes 3 and 7) and rat Pheochromocytoma (PC-12) cells (lanes 4 and 8):1-4. Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:200).
5-8. Anti-Neurexin 3α (extracellular) Antibody, preincubated with Neurexin 3α (extracellular) Blocking Peptide (#BLP-NR033).
- Expression of Neurexin 3α in rat and mouse brainImmunohistochemical staining of rat entorhinal cortex (A) and mouse striatum and lateral septum (B) using Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:400). A. Nrxn3α staining (green) appears in neuronal outlines in entorhinal cortex (arrow). B. Nrxn3α staining appeared in axonal processes in striatum (horizontal arrow) and in neuronal cell bodies in lateral septum (vertical arrows). DAPI is used as the counterstain (blue).
- (C)RYLRWDASTRSDLS, corresponding to amino acid residues 39-52 of rat Nrxn3α (Accession Q07310).
Accession (Uniprot) Number Q07310
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.